Author Correction: 7-Dehydrocholesterol is an endogenous suppressor of ferroptosis | Nature
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Subjects
- Cancer metabolism
- Cell death
The Original Article was published on 31 January 2024
Correction to: Nature https://doi.org/10.1038/s41586-023-06878-9 Published online 31 January 2024
In the originally published version of this article, the blank values in Extended Data Fig. 2a,b correspond to background fluorescence measurements of a stable commercial resazurin solution (AquaBluer Cell Viability Assay Solution, Multi-Target Pharmaceuticals, cat. no. 6015), diluted in complete culture medium. The values reported in the dataset are rounded versions of the direct fluorescence readouts obtained from a SpectraMax plate reader during each experiment, rounded to the nearest 50 fluorescence units. The subsequent experiments with a higher background value were acquired using a self-made resazurin solution, as indicated in the materials and methods, and recorded on the SPARK-Tecan plate reader. The blanks were defined as the average of three wells without rounding.
Following identification of the issue, we reanalysed the data to evaluate the potential impact of the blank values on our results, employing two distinct methodologies. First, we recalculated all values using a blank set to zero (see Figs. 1 and 2 in Supplementary Information), corresponding to 0% viability. Secondly, we normalised the data more stringently by defining the lowest measured condition — wild-type cells treated with 1 µM RSL3 or 10 µM FIN56 — as zero percent viability (see Figs. 3 and 4 in Supplementary Information). In both cases, the resulting plots fundamentally showed the same effects. These corrections do not affect the experiments and the study conclusions.
Supplementary information is available in the online version of this amendment.
Author information
Author notes- These authors contributed equally: Florencio Porto Freitas, Hamed Alborzinia, Ancély Ferreira dos Santos
Authors and Affiliations
- Rudolf Virchow Center for Integrative and Translational Bioimaging, University of Würzburg, Würzburg, Germany
Florencio Porto Freitas, Ancély Ferreira dos Santos, Alex Inague, Helene Nehring, Thamara N. Xavier da Silva, Zhiyi Chen, Sena Atici & José Pedro Friedmann Angeli
- Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH), Heidelberg, Germany
Hamed Alborzinia, Corinna Klein, Nesrine Aroua, Kamini Kaushal, Bettina Kast & Andreas Trumpp
- Division of Stem Cells and Cancer, German Cancer Research Center (DKFZ) and DKFZ-ZMBH Alliance, Heidelberg, Germany
Hamed Alborzinia, Corinna Klein, Nesrine Aroua, Kamini Kaushal, Bettina Kast & Andreas Trumpp
- Center of Membrane Biochemistry and Lipid Research, University Hospital and Faculty of Medicine Carl Gustav Carus of TU Dresden, Dresden, Germany
Palina Nepachalovich & Maria Fedorova
- Institute of Genetics, CECAD, University of Cologne, Cologne, Germany
Lohans Pedrera & Ana Garcia-Saez
- Department of Chemistry & Biomolecular Sciences, University of Ottawa, Ottawa, Ontario, Canada
Omkar Zilka, Emily L. Schaefer, Ifedapo Ekpo & Derek A. Pratt
- Instituto de Química, Universidade de Sao Paulo, Sao Paulo, Brazil
Alex Inague & Sayuri Miyamoto
- Institute of Metabolism and Cell Death, Helmholtz Zentrum München, Neuherberg, Germany
Svenja M. Lorenz, Sebastian G. Doll & Marcus Conrad
- Comprehensive Cancer Center Mainfranken, Universitätsklinikum Würzburg, Würzburg, Germany
Viktoria Kunz, Kurt Bommert & Ralf C. Bargou
- Department of Biochemistry and Molecular Biology, Theodor Boveri Institute, Biocenter, University of Würzburg, Würzburg, Germany
Werner Schmitz
- Institute of Pharmacy, Martin Luther University Halle Wittenberg, Halle, Germany
Aline Horling & Peter Imming
- Department of Biological Sciences, University of Denver, Denver, CO, USA
Ann M. Wehman
- Munroe-Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center, Omaha, NE, USA
Thiago C. Genaro-Mattos & Karoly Mirnics
- Faculty of Applied Sciences and Biotechnology, Shoolini University, Himachal Pradesh, India
Lokender Kumar
- Department of Physics, Colorado School of Mines, Golden, CO, USA
Judith Klein-Seetharaman
- School of Molecular Sciences, Arizona State University, Phoenix, AZ, USA
Judith Klein-Seetharaman
- Department of Pathology, University of Würzburg, Würzburg, Germany
Svenja Meierjohann
- Medizinische Klinik und Poliklinik IV, Ludwig Maximillian University, Munich, Germany
Isabel Weigand & Matthias Kroiss
- Institute of Experimental Cancer Research, University Hospital Ulm, Ulm, Germany
Georg W. Bornkamm
- Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil
Fernando Gomes & Luis Eduardo Soares Netto
- Department of Pharmacy, Ludwig Maximilian University of Munich, Munich, Germany
Manjima B. Sathian & David B. Konrad
- Department of Developmental Biology, Washington University in St. Louis, St. Louis, MO, USA
Douglas F. Covey
- Taylor Family Institute for Innovative Psych